pcib lentiviral expression vector  (Addgene inc)

Journal: Molecular Plant Pathology

Article Title: 6‐Hydroxynicotinic Acid From Cucumis melo Inhibits Prehaustorium Formation in Phelipanche aegyptiaca via Disruption of Auxin Signalling Pathway

doi: 10.1111/mpp.70137

Figure Lengend Snippet: Auxin inhibitors halt prehaustorium development in Phelipanche aegyptiaca . (A) The radicle tips in P. aegyptiaca after 3 days of treatment with gradient concentrations of p ‐chlorophenoxyisobutyric acid (PCIB) and 2,3,5‐triiodobenzoic acid (TIBA) (0.2, 0.1, 0.01 mM) combined with 2,6‐dimethoxy‐1,4‐benzoquinone (DMBQ) (0.01 mM) or indole‐3‐acetic acid (IAA) (0.1 mM). The corresponding control treatments included 0.01 mM DMBQ or 0.1 mM IAA alone. Red arrows indicate swollen radicle tips under various treatments. Scale bar: 500 μm. (B) Expansion rates of radicle tips. (C) Radicle length ( n = 21 seeds). (D) Endogenous IAA content. Data are presented as the mean ± SD (three biological replicates). Groups marked by different letters differ significantly ( p < 0.05) using one‐way ANOVA and Duncan's test. (E) Heatmap showing the hierarchical clustering of auxin‐related gene expression across six treatments: DMBQ + PCIB, DMBQ + TIBA, DMBQ, IAA + PCIB, IAA + TIBA and IAA. The raw data were normalised using Z‐score standardisation. The value ‘0’ represents the overall average expression level of each hormone across all samples. (F) Venn diagram showing the overlap of differentially expressed genes (DEGs) identified from pairwise comparisons: DMBQ + PCIB vs. DMBQ, DMBQ + TIBA vs. DMBQ, IAA + PCIB vs. IAA and IAA + TIBA vs. IAA. (G) KEGG enrichment analysis of 1106 DEGs common to the comparisons.

Article Snippet: The experimental groups received 0.1 mM IAA and 0.01 mM DMBQ mixed with 0.2, 0.1, or 0.01 mM PCIB (Shanghai Macklin Biochemical Co. Ltd.) or TIBA (Shanghai Macklin Biochemical Co. Ltd.) for 3 days.

Techniques: Control, Gene Expression, Expressing

Journal: Plants

Article Title: IAA-Mediated Haustorium Formation in Phelipanche aegyptiaca : Transcriptional Insights and Anti-Parasitic Strategies

doi: 10.3390/plants14111591

Figure Lengend Snippet: Effects of IAA and auxin inhibitor on the parasitic ability of Phelipanche aegyptiaca . ( A – C ) Growth status of melon seedlings inoculated with P. aegyptiaca , parasitic damage to their root systems, and developmental status of P. aegyptiaca under treatments with 15 μM PCIB, 0.1 mM IAA, and water (CK), respectively. ( D ) Total number of P. aegyptiaca attachments to the host roots in the three treatments (* p < 0.05). ( E ) Number of P. aegyptiaca attachments to the host roots at different developmental stages in the three treatments (* p < 0.05; ** p < 0.01). S7: P. aegyptiaca emergence; S6: P. aegyptiaca develops a young shoot > 2 cm but has not emerged; S5: Adventitious roots form at the nodules and connect with other roots < 1 cm. ( F ) Plant heights of the host plants under the three treatments (* p < 0.05; ** p < 0.01).

Article Snippet: The experimental design included three treatment groups: the 1 mM IAA aqueous solution treatment group, the 15 μM auxin activity inhibitor p -chlorophenoxyisobutyric acid (PCIB) [ ] (Macklin Biochemical Co., Ltd., Shanghai, China) aqueous solution treatment group, and the control group irrigated with only water.

Techniques: